Gene description for LGALS3BP
Gene name galectin 3 binding protein
Gene symbol LGALS3BP
Other names/aliases 90K
BTBD17B
CyCAP
M2BP
MAC-2-BP
TANGO10B
gp90
Species Homo sapiens
 Experiment description of studies that identified LGALS3BP in Melanoma cells

1
Experiment ID619
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameMALME-3M
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

2
Experiment ID621
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameSK-MEL-2
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

3
Experiment ID625
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameUACC-62
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

4
Experiment ID986
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 25261472   
OrganismHomo sapiens
Experiment descriptionComplex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment.
AuthorsLiang Y, Eng WS, Colquhoun DR, Dinglasan RR, Graham DR, Mahal LK.
Journal name J Biol Chem.
Publication year2014
SampleMelanoma cells
Sample nameSK-MEL-5
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140188: EV-METRIC:22%

5
Experiment ID1624
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyLC-MS/MS
Western blotting
EV-TRACK -

6
Experiment ID1625
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyLC-MS/MS
Western blotting
EV-TRACK -

7
Experiment ID1626
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyLC-MS/MS
Western blotting
EV-TRACK -

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